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CRISPR-Based Typing and Next-Generation Tracking Technologies.

Rodolphe Barrangou^1,2, Edward G Dudley²

¹Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina 27695;

Annual Review of Food Science and Technology

|January 17, 2016

Summary

This summary is machine-generated.

CRISPR-based genotyping offers a powerful alternative to traditional methods for accurately identifying and tracking bacteria in the food supply chain. This high-resolution technology aids in distinguishing beneficial microbes from harmful pathogens.

Keywords:

CRISPR cas epidemiology typing

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Area of Science:

Food microbiology
Genomics
Molecular biology

Background:

Bacteria are crucial in the food supply chain, impacting product safety, quality, and consumer health.
Accurate identification and typing of bacteria, from beneficial probiotics to harmful pathogens, are essential.
Current methods like pulsed-field gel electrophoresis (PFGE) are being succeeded by whole-genome sequencing (WGS).

Purpose of the Study:

To highlight the need for advanced bacterial identification tools.
To introduce CRISPR-based genotyping as a high-resolution alternative for bacterial typing.
To demonstrate the utility of CRISPR typing across diverse bacterial groups.

Main Methods:

Review of existing studies on CRISPR-based typing.
Comparison of CRISPR genotyping with traditional methods (PFGE) and WGS.
Application of CRISPR typing to various bacterial categories including starter cultures, probiotics, commensals, and pathogens.

Main Results:

CRISPR-based genotyping provides high-resolution insights into bacterial genome microevolution.
Studies show successful application of CRISPR typing for industrial starter cultures, probiotics, commensals, and pathogens.
CRISPR methods offer a practical approach for rapid tracking of food-associated microbes.

Conclusions:

CRISPR-based typing is a viable and powerful alternative for bacterial identification and tracking.
This technology facilitates a deeper understanding of bacterial populations in the food environment.
Emerging CRISPR methods enhance the ability to monitor diverse food-associated bacteria effectively.