Highly Fluorescent and Photostable Polymeric Nanofibers as Scaffolds for Cell Interfacing and Long-Term Tracking

Highly fluorescent polymeric nanofibers fabricated via electrospinning of PCL-DPP-PCL (photostable polycaprolactones-di(thiophene-2-yl)-diketopyrrolopyrrole-photostable polycaprolactones) and commercial PCL mixture show superior photostability and cytocompatibility for long-term tracking of cell-substrate interaction. As a proof of concept, these PCL-DPP-PCL nanofibers enable clear visualization of intricate cell-substrate interactions such as oligodendrocyte myelination.