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Updated: Mar 26, 2026

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications

Published on: February 7, 2021

Click-Crosslinked Injectable Gelatin Hydrogels.

Sandeep T Koshy^1,2,3, Rajiv M Desai^1,2, Pascal Joly^1,4

¹Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.

Advanced Healthcare Materials

|January 26, 2016

Summary

This summary is machine-generated.

Injectable ClickGel hydrogels mimic the cell environment, supporting cell viability and 3D growth. These biodegradable biomaterials offer promising applications for both in vitro and in vivo uses.

Keywords:

click chemistry degradable gelatin hydrogels injectable

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Published on: October 26, 2016

Area of Science:

Biomaterials Science
Tissue Engineering
Polymer Chemistry

Background:

Injectable hydrogels are crucial for tissue engineering and regenerative medicine.
Existing hydrogels often face challenges with cell encapsulation efficiency and in vivo performance.
Bioorthogonal click chemistry offers a rapid and specific method for hydrogel formation.

Purpose of the Study:

To develop novel injectable, cell-responsive gelatin hydrogels using bioorthogonal click chemistry.
To evaluate the cytocompatibility, cell morphology, and degradation properties of the developed hydrogels.
To assess the potential of these hydrogels as biomaterials for in vitro and in vivo applications.

Main Methods:

Synthesis of gelatin polymers functionalized with norbornene (GelN) or tetrazine (GelT) groups.
Formation of ClickGel hydrogels via spontaneous crosslinking of GelN and GelT.
Encapsulation of cells within the hydrogel matrix.
Assessment of cell viability, morphology, and proliferation.
In vivo injection and evaluation of hydrogel biodegradation.

Main Results:

ClickGel hydrogels formed rapidly and spontaneously upon mixing GelN and GelT.
High cell viability was maintained after encapsulation within the hydrogels.
Encapsulated cells adopted elongated 3D morphologies.
The hydrogels demonstrated biodegradation upon in vivo injection.

Conclusions:

ClickGel hydrogels represent a promising class of cell-responsive, injectable biomaterials.
The bioorthogonal click chemistry approach enables efficient cell encapsulation and supports cell function.
These hydrogels have significant potential for diverse in vitro and in vivo applications in regenerative medicine.