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Related Experiment Video

Updated: Mar 26, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System.

Christine M Thompson^1,2, Johnny Montes², Marc G Aucoin³

¹Department of Chemical Engineering, Ecole Polytechnique de Montreal, 2500 Chemin de Polytechnique, Montreal, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|January 29, 2016

Summary

This chapter details recombinant protein production using the baculovirus expression vector system (BEVS) in large bioreactors. It covers batch process methods from cell growth to product recovery, including advanced monitoring and control strategies.

Keywords:

Baculovirus Bioreactor Fed-batch High cell density Insect cell Large-scale Lytic system On-line monitoring Recombinant protein expression Serum-free medium

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Last Updated: Mar 26, 2026

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Area of Science:

Biotechnology
Molecular Biology
Bioprocess Engineering

Background:

The baculovirus expression vector system (BEVS) is a powerful tool for recombinant protein production.
Large-scale production in agitated bioreactors presents unique challenges for BEVS applications.

Purpose of the Study:

To provide a comprehensive overview of the methods for large-scale recombinant protein production using BEVS in agitated bioreactors.
To detail the critical stages of a batch process for efficient protein recovery.

Main Methods:

Host cell cultivation and virus stock amplification and quantification.
Bioreactor preparation, operation, and the infection process.
Product harvesting and primary separation techniques, including on-line monitoring and control.

Main Results:

Detailed methodologies for each stage of the BEVS batch process are presented.
The importance of advanced on-line monitoring and bioreactor control for process success is highlighted.

Conclusions:

Successful large-scale recombinant protein production using BEVS requires careful optimization of each process step.
Integration of advanced monitoring and control enhances overall process efficiency and product yield.