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Vaccine Production

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

Vaccinations

Vaccinations

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Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the...

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Related Experiment Video

Updated: Mar 26, 2026

Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

Production of Virus-Like Particles for Vaccination.

Christine M Thompson^1,2, Marc G Aucoin³, Amine A Kamen^2,4

¹Department of Chemical Engineering, Ecole Polytechnique de Montreal, 2500, Chemin de Polytechnique, Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|January 29, 2016

Summary

Virus-like particles (VLPs) production is successful in baculovirus expression vector systems using insect cells. These VLPs show promise as vaccine candidates and therapeutic delivery agents, with scalable production methods being developed.

Keywords:

Baculovirus Insect cells VLP Viral vector Virus-like particle

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Area of Science:

Biotechnology
Virology
Vaccine Development

Background:

The baculovirus expression vector system (BEVS) in insect cells enables the production of diverse virus-like particles (VLPs).
VLPs are increasingly recognized for their potential applications in medicine.

Purpose of the Study:

To highlight the successful production of various VLPs using the BEVS/insect cell system.
To discuss the ongoing efforts in scaling up VLP production for potential therapeutic and vaccine use.
To emphasize the suitability of insect cells for large-scale VLP manufacturing.

Main Methods:

Utilizing the baculovirus expression vector system (BEVS) for VLP synthesis in insect cell hosts.
Focusing on the production and scale-up strategies for VLP manufacturing.
Investigating VLPs as vaccine candidates and therapeutic delivery vehicles.

Main Results:

Successful generation of a wide array of virus-like particles (VLPs) has been demonstrated.
The BEVS/insect cell system provides a scalable platform for VLP production.
VLPs are being actively explored for their roles in vaccines and therapeutics.

Conclusions:

The BEVS/insect cell system is a validated and scalable method for producing diverse VLPs.
VLPs produced via this system hold significant potential as vaccine candidates and therapeutic delivery agents.
Ongoing research focuses on optimizing production and exploring the full therapeutic potential of VLPs.