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Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

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Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
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Updated: Mar 26, 2026

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
07:03

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

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Breast Cancer Stem Cell Isolation.

Xuanmao Jiao1, Albert A Rizvanov2, Massimo Cristofanilli1

  • 1Department of Cancer Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA, 19107, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2016
PubMed
Summary
This summary is machine-generated.

Cancer stem cells drive tumor growth and recurrence. This protocol details methods for identifying and purifying these critical cells in breast cancer research, enabling further functional studies.

Keywords:
Breast cancer stem cellsCirculating tumor cellsFluorescence-activated cell sorting (FACS)Mammosphere formationTransplantation assays

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Area of Science:

  • Oncology
  • Cancer Biology
  • Stem Cell Research

Background:

  • Tumors exhibit significant cellular heterogeneity.
  • A small subpopulation of tumor cells, known as cancer stem cells (CSCs), possesses tumor-initiating capabilities.
  • CSCs are theorized to originate from normal stem or progenitor cells that acquire mutations, leading to aberrant differentiation.

Purpose of the Study:

  • To provide a foundational protocol for the identification and purification of breast cancer stem cells.
  • To equip researchers with essential techniques for CSC isolation and characterization.
  • To facilitate further functional assays for comprehensive CSC research.

Main Methods:

  • Detailed protocol for the identification of breast cancer stem cells.
  • Methods for the purification of CSCs from tumor samples.
  • Guidelines for subsequent functional assays to study CSC behavior.

Main Results:

  • Established a reliable method for CSC identification and purification.
  • Demonstrated the utility of the protocol in isolating viable CSCs.
  • Enabled downstream functional analyses crucial for understanding CSC roles.

Conclusions:

  • Accurate identification and purification of CSCs are paramount for effective cancer research.
  • This protocol serves as a valuable resource for researchers studying breast cancer stem cells.
  • The methodology supports advancements in understanding tumor progression and recurrence driven by CSCs.