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[SYNTHESIS OF PHYTOHORMONES BY NOCARDIA VACCINII IMV B-7405--PRODUCER OF SURFACTANTS]
Mikrobiolohichnyi Zhurnal (Kiev, Ukraine : 1993)
|February 3, 2016
Summary
Nocardia vaccinii can produce phytohormones and surfactants using waste oil. This study explored optimizing their synthesis using different carbon sources for potential industrial applications.
Area of Science:
- Microbiology
- Biotechnology
- Plant Science
Background:
- Nocardia vaccinii IMV B-7405 is a known surfactant producer.
- Phytohormones regulate plant growth and development.
- Microbial synthesis offers a sustainable route for producing valuable compounds.
Purpose of the Study:
- To investigate phytohormone (auxins, cytokinins, abscisic acid) synthesis by Nocardia vaccinii.
- To evaluate the impact of different carbon sources (glycerol, refined sunflower oil, waste oil) on phytohormone and surfactant production.
- To assess the feasibility of utilizing industrial waste for microbial synthesis.
Main Methods:
- Cultivation of Nocardia vaccinii on various carbon sources.
- Extraction of phytohormones from culture supernatants using ethylacetate and n-butanol.
- Purification via thin-layer chromatography.
- Quantitative determination using a scanning Sorbfil spectrodensitometer.
Main Results:
- Refined sunflower oil supported maximum auxins (245-770 µ/l) and cytokinins (134-348 µl) production, alongside 1.8 g/l surfactant.
- Waste oil cultivation yielded lower phytohormone levels (23-84 µ/l auxins, 16-90 µ/l cytokinins) but increased surfactant yield (2.3-2.6 g/l).
- Abscisic acid levels were consistently low (2-12 µ/l) and independent of the carbon source.
Conclusions:
- Oil-containing industrial waste can be utilized for simultaneous surfactant and phytohormone synthesis.
- Cultivation conditions significantly influence the yield and profile of microbial products.
- Further research is needed to optimize conditions for enhanced biological properties.
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