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Updated: Mar 26, 2026
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Published on: May 2, 2018
[EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING
Aim:
Study the effect of lactobacilli exopolysaccharides (EPS)on cytokine and phagocyte activity in vitro and in mice organism during modelling of an infectious process.
Materials And Methods:
Lactobacillus delbrueckii subsp. delbrueckii B-1596 (laksaran 1596), L. delbrueckii B-1936 (laksaran 1936) and L. delbrueckii ssp. bulgaricus (laksaran Z) were used in the study. EPS were administered into white mice 1 hour after the Staphylococcus aureus 209-P infection. Index of phagocyte completion and index of killing activation (IKA) were calculated during phagocyte activity study. IL-1α, TNF-α, IFN-γ and IL-4 cytokine content was determined in blood sera and macrophage supernatants.
Results:
Laksaran 1596, 1936 and Z had ambiguous effect on cytokine production. Laksaran: Z and 1936, 6 hours after mice infection increased IL-1 content in blood sera. Laksaran Z had the most pronounced effect on macrophages, resulting in an increase of active macrophages, facilitating increased digestion of S. aureus 209-P and IKA increase, stimulated cytokine production.
Conclusion:
The results obtained allow to speak about a possibility of using laksaran Z as a prophylaxis immune modulating preparation for correction of animal cytokine status.
