[STUDY OF COLONIZATION PROCESSES AND PERSISTENCE OF MICROORGANISMS IN ARTIFICIAL MATERIALS FOR MEDICAL USE].

Area of Science:

• Biomaterials Science
• Microbiology
• Surface Science

Background:

• Polymer materials are widely used in medical devices, including removable dental prostheses.
• Understanding microbial colonization and persistence on these materials is crucial for patient safety and device longevity.

Purpose of the Study:

• To investigate the processes of microbial colonization and persistence on polymer materials intended for medical applications.
• To analyze the interaction dynamics between specific bacterial species and polymer surfaces over extended periods.

Main Methods:

• Polymer samples (polyurethane and acrylic) for dental prostheses were incubated with Pseudomonas aeruginosa and Staphylococcus aureus.
• Incubation occurred in Luria-Bertani broth at 37°C for durations up to 3 months.
• Scanning electron microscopy (SEM) was used to examine biofilm formation and material surface changes, preserving native sample structures.

Main Results:

• Electron microscopy revealed distinct stages of bacterial interaction with medical plastic surfaces.
• Biofilm formation was observed on abiotic polymer surfaces.
• Biodestructive changes in the plastic materials were evident, suggesting material degradation.

Conclusions:

• The study provides insights into the stages of microbial interaction and biofilm development on medical polymers.
• The developed experimental model using SEM can serve as a foundation for future research on combating biofilms.
• The findings raise concerns about the potential for prolonged persistence of pathogenic microorganisms on artificial prostheses.