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Updated: Mar 26, 2026
Isolation of Epithelial Cells from Human Dental Follicle
Published on: November 5, 2021
Expression of Pattern Recognition Receptors in Epithelial Cells Around Clinically Healthy Implants and Healthy Teeth
Roberta Calcaterra1, Michele Di Girolamo, Concetta Mirisola
1*Researcher, Department of Social Dentistry, National Institute for Health, Migration and Poverty, Rome, Italy. †Assistant Professor, Department of Clinical Sciences and Translational Research, School of Dentistry, University of Rome, Torvergata, Italy. ‡Director, Department of General Direction, National Institute for Health, Migration, and Poverty, Rome, Italy. §Director, Department of Social Dentistry, National Institute for Health, Migration and Poverty-Associate Professor School of Dentistry, University of Rome Tor vergata, Italy.
Background:
Gingival epithelial cells have a pivotal role in the recognition of microorganisms and damage-associated molecular pattern molecules and in the regulation of the immune response. The investigation of the behavior of Toll-like receptors (TLRs) and nucleotide oligomerization domain (NOD) like receptors (NLRs) around a healthy implant may help to address the first step of periimplantitis pathogenesis.
Purpose:
To investigate by quantitative real-time polymerase chain reaction, the mRNA expressions of TLR2, TLR3, TLR4, TLR5, TLR6, TLR9, NOD1, NOD2, and NLRP3 from gingival epithelial cells of the sulcus around healthy implants and around healthy teeth.
Materials And Methods:
Two types of implant-abutment systems with tube-in-tube interface were tested. After 6 months of implant restoration, gingival epithelial cells were obtained from the gingival sulcus around the implants and around the adjacent teeth of 10 patients.
Results:
Our results did not reach statistical significance among the mRNA expressions of TLR2, TLR3, TLR4, TLR5, TLR6, TLR9, NOD1, NOD2, and NLRP3 in epithelial cells around the implant versus around natural teeth.
Conclusion:
This study shows that the implant-abutment systems tested did not induce an immune response by the surrounding epithelial cells at 6 months since their positioning, as well as in the adjacent clincally healthy teeth.
