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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Related Experiment Video

Updated: Mar 26, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Beyond Conventional Quantum Dots

Masaru Kuno¹, Liberato Manna², Narayan Pradhan³

¹Department of Chemistry and Biochemistry, University of Notre Dame, 251, Nieuwland Science Hall, Notre Dame, IN, 46556, USA. mkuno@nd.edu.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry

|February 5, 2016

Summary

No abstract available in PubMed .

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