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Heterokaryon Technique for Analysis of Cell Type-specific Localization
Published on: March 11, 2011
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Data from comprehensive analysis of nuclear localization signals.
Ryosuke Yamagishi1, Hiroki Kaneko2
1Department of Integrated Sciences in Physics and Biology, College of Humanities and Sciences, Nihon University, 3-25-40 Sakurajousui, Setagaya, Tokyo 156-8550, Japan.
Data in Brief
|February 11, 2016
Summary
This study presents data on mammalian nuclear localization signals (NLSs) from UniProt, detailing their sequences, lengths, and distribution across proteins for structural analysis.
Area of Science:
- Molecular Biology
- Bioinformatics
- Proteomics
Background:
- Nuclear localization signals (NLSs) are crucial for protein transport into the cell nucleus.
- Understanding NLS dynamics is key to cellular function and dysfunction.
Purpose of the Study:
- To compile and analyze a comprehensive dataset of mammalian NLSs.
- To provide data on NLS sequences, lengths, and distribution from the UniProt database.
Main Methods:
- Extraction of NLSs explicitly annotated in UniProt with defined evidence codes.
- Analysis of 1364 NLSs identified within 1186 unique proteins.
- Cataloging NLS count per protein, sequence length, and overall distribution patterns.
Main Results:
- A substantial dataset of 1364 NLSs from 1186 mammalian proteins was curated.
- Data includes specific UniProt IDs, NLS sequence lengths, and their frequency distribution.
- The analysis covers a broad range of NLSs irrespective of their conformational states.
Conclusions:
- The compiled data offers a valuable resource for studying NLS structure-function relationships.
- This comprehensive dataset facilitates further research into nuclear import mechanisms.
- The findings support a deeper understanding of protein trafficking and localization in mammalian cells.
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