Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

28.6K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.6K
Experimental RNAi02:15

Experimental RNAi

8.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.3K
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

107
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
107
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.0K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

5.2K
5.2K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026
Same author

3D Bioprinting of High-Grade Serous Ovarian Cancer Cells: Workflow for Gelatin-Based Bioink Formulation with Hyaluronic Acid and Printability Assessment.

ACS applied bio materials·2026
Same author

On the Prediction of pDNA Productivity Across Diverse Bioprocesses Using Ensemble Hybrid Models.

Biotechnology and bioengineering·2026
Same author

Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.

ACS applied nano materials·2026
Same author

Drug Delivery from Nanogel Formulations: Mathematical Modeling of Different Release Profiles and Conditions.

Molecular pharmaceutics·2025
Same author

3D Printed Microfluidic Chromatographic Column for Fast Downstream Processing Development.

Biotechnology journal·2025

Related Experiment Video

Updated: Mar 25, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.1K

Small interfering RNA delivery through positively charged polymer nanoparticles.

Luca Dragoni1, Raffaele Ferrari, Monica Lupi

  • 1Department of Chemistry, Materials and Chemical Engineering, Politecnico di Milano, Via Luigi Mancinelli 7, 20131, Milan, Italy.

Nanotechnology
|February 19, 2016
PubMed
Summary

Researchers developed biocompatible nanoparticles to deliver small interfering RNA (siRNA) for genetic disease treatment. These nanoparticles successfully delivered functional siRNA into cancer cells, inhibiting target gene expression.

More Related Videos

Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
09:36

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Published on: February 5, 2019

9.5K

Related Experiment Videos

Last Updated: Mar 25, 2026

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.1K
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
09:36

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Published on: February 5, 2019

9.5K

Area of Science:

  • Biotechnology
  • Materials Science
  • Molecular Biology

Background:

  • Small interfering RNA (siRNA) holds promise for treating genetic diseases like cancer and diabetes.
  • siRNA's large size and instability hinder its cellular uptake and therapeutic application.
  • Effective delivery systems are crucial for siRNA-based therapies.

Purpose of the Study:

  • To synthesize biocompatible, positively charged nanoparticles (NPs) for siRNA delivery.
  • To demonstrate the NPs' ability to load and deliver intact siRNA into cancer cells.
  • To validate the gene silencing efficacy of siRNA delivered by the NPs.

Main Methods:

  • Utilized a two-step synthesis involving ring-opening polymerization (ROP) and emulsion free radical polymerization (EFRP).
  • Characterized NPs, tuning size and surface charge by adjusting synthesis parameters.
  • Demonstrated siRNA loading, intracellular delivery via NP biodegradation, and gene silencing in GFP-expressing cells.

Main Results:

  • Successfully synthesized tunable, biocompatible, positively charged NPs.
  • Confirmed efficient loading and delivery of undamaged siRNA into cancer cell cytoplasm.
  • Showcased successful target gene inhibition mediated by NP-delivered siRNA.

Conclusions:

  • Developed a novel nanoparticle system for effective siRNA delivery.
  • Demonstrated the potential of these NPs for targeted gene silencing in cancer therapy.
  • Highlighted the tunable biodegradation kinetics of NPs for controlled siRNA release.