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Updated: Mar 25, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Multiplexed Engineering in Biology.

Jameson K Rogers¹, George M Church¹

¹Wyss Institute for Biologically Inspired Engineering, Harvard University, 3 Blackfan Circle, Boston, MA 02115, USA.

Trends in Biotechnology

|February 22, 2016

Summary

This summary is machine-generated.

Biological engineering accelerates drug and chemical production by optimizing the design-build-test cycle. A new multiplexed testing method significantly enhances evaluation capacity, boosting biobased product development throughput.

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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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Published on: April 14, 2015

Design and Use of Multiplexed Chemostat Arrays

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Published on: February 23, 2013

The MultiBac Protein Complex Production Platform at the EMBL

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Published on: July 11, 2013

Area of Science:

Synthetic biology
Biotechnology
Genetic engineering

Background:

Biotechnology relies on genetic engineering to create high-yield cells for drug and chemical production.
Current biological engineering faces bottlenecks in evaluating numerous genetic variants due to limited testing capacity.

Purpose of the Study:

To analyze biological engineering through the design-build-test cycle framework.
To introduce a multiplexed solution for the 'test' phase to overcome evaluation limitations.

Main Methods:

Evaluation of the design-build-test cycle in biological engineering.
Description of a novel multiplexed approach for the 'test' step.

Main Results:

Multiplexing has revolutionized the design and build phases of biological engineering.
The proposed multiplexed test step significantly increases evaluation throughput.

Conclusions:

A fully multiplexed engineering cycle is achievable with advancements in the test step.
The new approach can enhance biobased product development throughput by up to a millionfold.