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Updated: Aug 15, 2026
Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats
Published on: October 1, 2007
Local adoptive transfer of delayed-type hypersensitivity to tuberculin from M. bovis (BCG) infected mice
B V Nikonenko1, M B Mezhlumova, A S Apt
1Central Research Institute for Tuberculosis, Ministry of Public Health of the USSR, Moscow.
Immunological properties of the cells mediating delayed-type hypersensitvity to tuberculin and genetic requirements of this reaction have been studied by the method of local adoptive transfer. Peritoneal cells from BCG-immunized mice transferred the reaction into unprimed recipients without any genetic restriction. In contrast, nonadherent peritoneal cells transferred the reaction only in H-2-compatible donor-recipient strain combinations. The use of H-2 recombinant strains has shown that delayed-type hypersensitivity to tuberculin in mice is restricted by the I-A locus. Monoclonal antibody against I-Ak beta abrogated (without complement) the transfer of the reaction to CBA recipients. The phenotype of nonadherent cells, transferring the reaction has been shown to be Thy-1+, Lyt-1+, 2-, L3T4+.
Immunological properties of the cells mediating delayed-type hypersensitvity to tuberculin and genetic requirements of this reaction have been studied by the method of local adoptive transfer. Peritoneal cells from BCG-immunized mice transferred the reaction into unprimed recipients without any genetic restriction. In contrast, nonadherent peritoneal cells transferred the reaction only in H-2-compatible donor-recipient strain combinations. The use of H-2 recombinant strains has shown that delayed-type hypersensitivity to tuberculin in mice is restricted by the I-A locus. Monoclonal antibody against I-Ak beta abrogated (without complement) the transfer of the reaction to CBA recipients. The phenotype of nonadherent cells, transferring the reaction has been shown to be Thy-1+, Lyt-1+, 2-, L3T4+.
