Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

5.1K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
5.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.7K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Determination of Crystal Structures01:29

Determination of Crystal Structures

66
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
66
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.9K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.9K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>In-situ</i> structural and electrical conductivity characterization of Sr<sub>2</sub> <i>M</i>MoO<sub>6-δ</sub> double perovskite solid oxide fuel cell anode materials.

ACS applied energy materials·2026
Same author

Morphological and property studies of charged pentablock terpolymer gels containing an aliphatic oil.

Journal of colloid and interface science·2026
Same author

Plasma-Enhanced Atomic Layer Deposition Synthesis of Nanolayered Molybdenum Diselenide (MoSe<sub>2</sub>) Thin Films for Nanoelectronics.

ACS omega·2026
Same author

Uptake kinetics and photocrosslinking of poly(ethylene glycol) diacrylate in the nanostructure of a self-assembled sulfonated pentablock polymer.

Journal of colloid and interface science·2026
Same author

Valence-free open nanoparticle superlattices.

Nature communications·2026
Same author

Direct Imaging of Hydrogen-Driven Dislocation and Strain Field Evolution in a Stainless Steel Grain.

Advanced materials (Deerfield Beach, Fla.)·2025
Same journal

Launching a new era for Short Communications in Journal of Synchrotron Radiation.

Journal of synchrotron radiation·2026
Same journal

Sagittal collimating diaboloid: a new grazing-incidence mirror surface for higher-throughput resonant inelastic X-ray scattering spectrometers.

Journal of synchrotron radiation·2026
Same journal

Synchrotron X-ray tomography and spectroscopy in numismatics: disclosing counterfeit practices in medieval silver coins.

Journal of synchrotron radiation·2026
Same journal

The Big Data Science Center at the Shanghai Synchrotron Radiation Facility: the architecture of the superfacility.

Journal of synchrotron radiation·2026
Same journal

A robotic and high-throughput X-ray micro-computed tomography workflow.

Journal of synchrotron radiation·2026
Same journal

Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.

Journal of synchrotron radiation·2026
See all related articles

Related Experiment Video

Updated: Mar 25, 2026

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets
06:26

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

7.7K

Transmission X-ray scattering as a probe for complex liquid-surface structures.

Masafumi Fukuto1, Lin Yang1, Dmytro Nykypanchuk2

  • 1National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA.

Journal of Synchrotron Radiation
|February 27, 2016
PubMed
Summary
This summary is machine-generated.

Scanning transmission X-ray scattering offers a new way to study complex liquid surface structures. This method can map local order and domain shapes, crucial for developing advanced functional materials.

Keywords:
SAXSWAXSair/water interfaceliquid surface scatteringtransmission X-ray scattering

More Related Videos

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

9.6K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.5K

Related Experiment Videos

Last Updated: Mar 25, 2026

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets
06:26

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

7.7K
Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

9.6K
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

13.5K

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Nanotechnology

Background:

  • Complex self-assembly systems are essential for creating functional materials.
  • Probing local structure and heterogeneities at liquid surfaces is critical for controlling self-assembly.
  • Traditional X-ray scattering methods have limitations in spatially resolving lateral heterogeneities on liquid surfaces.

Purpose of the Study:

  • To investigate the feasibility of using scanning transmission X-ray scattering (TSAXS/TWAXS) for characterizing heterogeneous structures at liquid surfaces.
  • To assess the capability of TSAXS/TWAXS in simultaneously probing local intermolecular structures and domain morphologies.
  • To evaluate TSAXS/TWAXS for analyzing Langmuir films on water meniscus.

Main Methods:

  • Performed transmission small- and wide-angle X-ray scattering (TSAXS/TWAXS) experiments.
  • Studied Langmuir films of gold nanoparticles and fluorinated fatty acids on a water meniscus.
  • Analyzed diffraction patterns from multiple spots to extract surface shape and monolayer coverage.

Main Results:

  • Observed first-order diffraction rings in TSAXS and TWAXS patterns for both nanoparticle and fatty acid monolayers.
  • Successfully extracted information about the water meniscus shape and ordered-monolayer coverage.
  • Demonstrated the ability to spatially resolve structural features using the scanning probe technique.

Conclusions:

  • Scanning-probe TSAXS/TWAXS is a feasible approach for characterizing heterogeneous structures at liquid surfaces.
  • This technique enables simultaneous probing of local structure and domain morphology.
  • Advancements in X-ray beam technology further support the potential of this method for materials characterization.