Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.7K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A continuous-discrete model of cell contraction incorporating actin and intermediate filaments.

iScience·2026
Same author

Smart event-triggered MINFLUX microscopy to catch and follow rare events.

Nature communications·2026
Same author

Protocol for microfluidic-based high-precision general polarization fluorescence microscopy of lipid packing in membrane vesicles.

STAR protocols·2026
Same author

Resolving the mechanical paradox of myelination.

Communications biology·2026
Same author

Metabolic profiling of steatotic liver disease by fluorescence lifetime imaging microscopy.

Communications medicine·2026
Same author

Lightweight CycleGAN models for cross-modality image transformation and experimental quality assessment in fluorescence microscopy.

Biomedical optics express·2026
Same journal

Monolithic Axial InGaAs Quantum Dot Emitters in GaAs-Based Nanowires via Sb-Mediated Facet Engineering.

Nano letters·2026
Same journal

Electrical Imaging of DNA Substructures Using Quasi-Static Nanopore Scanning.

Nano letters·2026
Same journal

Structural Basis of Hemoglobin Amyloid Fibrils Revealed by cryo-EM and Molecular Dynamics Simulations.

Nano letters·2026
Same journal

Rashba-Related Spin-Selective Effect in 2D Chiral Perovskites with Achiral Organic Cation Spacers.

Nano letters·2026
Same journal

Visualizing Superconducting Gap Modulation Induced by Pair-Breaking Scattering Interference in Bulk FeSe.

Nano letters·2026
Same journal

Generalized Geometric Phase for Coupled Meta-Atoms.

Nano letters·2026
See all related articles

Related Experiment Video

Updated: Mar 25, 2026

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
08:10

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Published on: October 6, 2019

7.0K

Super-Resolved Traction Force Microscopy (STFM).

Huw Colin-York, Dilip Shrestha, James H Felce

  • 1Department of Biological Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02142, United States.

Nano Letters
|March 1, 2016
PubMed
Summary
This summary is machine-generated.

We enhanced traction force microscopy (TFM) using STED microscopy for higher resolution force measurements in cell biology. This new STED-TFM technique achieves over 5x greater sampling, enabling nano-scale force analysis.

Keywords:
Super-resolution microscopyactin cytoskeletonmechanobiologytraction force microscopy

More Related Videos

Traction Force Microscopy to Study B Lymphocyte Activation
09:28

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

7.0K
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

7.6K

Related Experiment Videos

Last Updated: Mar 25, 2026

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform
08:10

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

Published on: October 6, 2019

7.0K
Traction Force Microscopy to Study B Lymphocyte Activation
09:28

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

7.0K
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration
10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Published on: October 13, 2019

7.6K

Area of Science:

  • Cell Biology
  • Biophysics
  • Microscopy

Background:

  • Measuring cellular forces is crucial but challenging.
  • Conventional traction force microscopy (TFM) has limitations in spatial resolution.
  • Advancements are needed to probe forces at finer scales.

Purpose of the Study:

  • To improve the spatial resolution and accuracy of force reconstruction in TFM.
  • To introduce STED-TFM as a novel technique for nanoscale force mapping.
  • To demonstrate the enhanced capabilities of STED-TFM compared to conventional TFM.

Main Methods:

  • Utilized STED (Stimulated Emission Depletion) microscopy to enhance TFM.
  • Developed STED-TFM (STFM) for higher spatial resolution force measurements.
  • Employed computer simulations and an activating RBL cell model system for validation.

Main Results:

  • Achieved over 5-fold higher sampling of cellular forces compared to conventional TFM.
  • Enabled force measurements at the nano-scale, a significant improvement over the micron-scale.
  • Demonstrated the enhanced resolution and accuracy of STFM through simulations and experimental models.

Conclusions:

  • STED-TFM significantly advances the capability to measure cellular forces with unprecedented spatial resolution.
  • The nano-scale force mapping achieved by STFM opens new avenues in cell biology research.
  • STFM provides a more detailed understanding of cell-generated forces.