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Lineage analysis by microsatellite loci deep sequencing in mice
Tao Luo1, Xionglei He1,2, Ke Xing1
1State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences, Sun Yatsen University, Guangzhou, China.
Molecular Reproduction and Development
|March 3, 2016
Summary
This study introduces a new method for lineage analysis using genome-wide microsatellite mutations and next-generation sequencing. This approach effectively traces cell progeny and developmental origins in multicellular organisms.
Area of Science:
- Developmental Biology
- Genetics
- Genomics
Background:
- Lineage analysis identifies cell progeny from a single progenitor.
- It is crucial for understanding development and cancer.
- Existing methods may have limitations in complex tissues.
Purpose of the Study:
- To develop a novel and effective method for lineage analysis.
- To utilize genome-wide microsatellite loci and next-generation sequencing.
- To construct phylogenetic trees based on somatic mutations.
Main Methods:
- Sequence capture and next-generation sequencing were employed.
- Genome-wide mononucleotide and dinucleotide microsatellite loci were identified.
- Phylogenetic trees were constructed using somatic indel mutations.
Main Results:
- Somatic indel mutations at microsatellite loci were unique enough to distinguish samples.
- Samples from different mice were parsed into distinct groups on lineage trees.
- Tissues with common developmental origins (e.g., liver and stomach from endoderm) clustered together.
- Kidney samples (mesoderm) formed a separate clade.
Conclusions:
- This genome-wide approach provides non-invasive lineage analysis.
- It relies on mutations accumulating in somatic cells.
- The method is effective for opaque multicellular organisms.
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