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Updated: Mar 24, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
Proteomic Analysis of Protein Posttranslational Modifications by Mass Spectrometry
Danielle L Swaney1, Judit Villén1
1Department of Genome Sciences, University of Washington, Seattle, Washington 98195.
Abstract:
The addition of posttranslational modifications (PTMs) to proteins is an influential mechanism to temporally control protein function and ultimately regulate entire cellular processes. Most PTMs are present at low stoichiometry and abundance, which limits their detection when analyzing whole cell lysates. PTM purification methods are thus required to comprehensively characterize the presence and dynamics of PTMs using mass spectrometry-based proteomics approaches. Here we describe several of the most influential PTMs and discuss the fundamentals of proteomics experiments and PTM purification methods.
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