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Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae
Hirotoshi Endo1,2,3, Megumi Yoshida3, Toshiki Uji4
1Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Japan.
Abstract:
Of the three dominant marine microalgal groups, dinoflagellates and diatoms can undergo genetic transformation; however, no transformation method has been established for haptophytes to date. Here, we report the first stable genetic transformation of a coccolithophore, Pleurochrysis carterae, by means of polyethylene glycol (PEG)-mediated transfer of a bacterial hygromycin B-resistance gene. Together with the novel transient green fluorescent protein (GFP) expression system, this approach should facilitate further molecular-based research in this phylum.
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