[Clinical and Biological Character in Mouse Models for Middle East Respiratory Syndrome Generated by Transduction with Different Doses of DPP4 Molecule]
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Abstract:
In this study, we evaluated the difference ot biological characteristics in the MERS-CoV infected mice model in prior to transduction with different dosage of human DPP4. Firstly, we transduced different dosage of DPP4 (high or low) into mice, and then challenged them with MERS-CoV in order to establish the model. After establishment of mice model, we observed the clinical signs of disease, virus replication, immunopathogenesis and antibody response. The results indicated that the infected mice showed typical pneumonia, virus replication, histological lesions, and neutralizing antibody production. Moreover, the high dosage group was superior to the low dosage group. Fourteen days after infection, the specific antibody to virus structural protein and neutralizing antibody were analyzed, the high dosage group induced higher level antibody. In summary, the MERS-CoV infected mice model were established prior transduction with DPP4, and the level of DPP4 influenced the clinical signs of disease, virus replication and antibody response in this model.
Insights
This study established a MERS-CoV infected mice model using human DPP4 transduction. Higher DPP4 dosage enhanced disease characteristics and antibody response in MERS-CoV mice models.
Area of Science:
- Virology
- Immunology
- Animal Models
Context:
- Middle East Respiratory Syndrome Coronavirus (MERS-CoV) poses a significant public health threat.
- Understanding MERS-CoV pathogenesis and host response is crucial for developing effective countermeasures.
- The dipeptidyl peptidase 4 (DPP4) receptor is essential for MERS-CoV entry into host cells.
Purpose:
- To evaluate the impact of different human DPP4 dosages on the biological characteristics of a MERS-CoV infected mice model.
- To establish a reliable MERS-CoV mice model for studying disease progression and immune responses.
- To investigate how DPP4 levels influence viral replication, clinical signs, and antibody production.
Summary:
- Mice were transduced with high or low dosages of human DPP4 before MERS-CoV challenge to establish an infection model.
- The study observed clinical signs, viral replication, immunopathogenesis, and antibody responses in the established model.
- Results showed that higher DPP4 dosage led to more pronounced pneumonia, increased viral replication, and a stronger neutralizing antibody response.
Impact:
- This study provides a refined MERS-CoV mice model for preclinical research.
- The findings highlight the critical role of DPP4 levels in modulating MERS-CoV infection severity and host immunity.
- This model can aid in evaluating antiviral therapies and vaccine efficacy against MERS-CoV.
