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Effect of ozone on colon anastomoses in rat peritonitis model
Tuğrul Çakır1, Arif Aslaner1, Seçkin Özgür Tekeli2
1Department of General Surgery, Antalya Training and Research Hospital, Antalya, Turkey.
Purpose:
To investigate the effects of medical ozone theraphy on the colon anastomosis of peritonitis model in rats.
Methods:
Eighteen rats were randomly assigned into three equal groups; control, cecal punctuation and colon anastomosis and ozone theraphy. Sepsis was performed with a cecal punctuation in groups 2 and 3. The medical ozone theraphy was administered intraperitonealy for three weeks in group 3 while the other rats received saline injection. At the twenty second day serum were obtained for TNF-α and IL-1β, the colonic burst pressures were measured and colonic tissue samples were obtained for MDA and MPO levels. Histolopatological examination was evaluated with H&E stain, and Ki-67, IL-1β and the VEGF immunostaining densities were also compared.
Results:
Intraperitoneal ozone administration reversed TNF-α, IL-1β, MDA and MPO levels and the colonic burst pressures. There was also a significant difference at immunostaining densities of histopathological examination.
Conclusion:
Medical ozone therapy may contribute to tissue healing by affecting the proliferation and the vascularization thus has benefits on colonic anastomosis at peritonitis in rats.
