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Updated: Mar 24, 2026

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
Published on: January 16, 2021
Radiation damage and derivatization in macromolecular crystallography: a structure factor's perspective
Robin L Owen1, Darren A Sherrell1
1Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, England.
Abstract:
During, or even after, data collection the presence and effects of radiation damage in macromolecular crystallography may not always be immediately obvious. Despite this, radiation damage is almost always present, with site-specific damage occurring on very short time (dose) scales well before global damage becomes apparent. A result of both site-specific radiation damage and derivatization is a change in the relative intensity of reflections. The size and approximate rate of onset of X-ray-induced transformations is compared with the changes expected from derivatization, and strategies for minimizing radiation damage are discussed.
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