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Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
Published on: February 7, 2025
Imidazolium-tagged glycan probes for non-covalent labeling of live cells
David Benito-Alifonso1, Shirley Tremell, Joanna C Sadler
1School of Chemistry, University of Bristol, Cantock's Close, BS8 1TS, UK. M.C.Galan@bristol.ac.uk.
Abstract:
Selective, bioorthogonal and fast labeling of glycoconjugates in living cells is a major challenge for synthetic and cellular biology. Here we report the use imidazolium tagged-mannosamine derivative (ITag-Man) for the non-covalent, rapid and site-specific labeling of sialic acid containing glycoproteins using commercial N-nitrilotriacetate fluorescent reagents in a range of cell lines.
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