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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

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Immunosensors in Clinical Laboratory Diagnostics.

Celine I L Justino¹, Armando C Duarte², Teresa A P Rocha-Santos²

¹CESAM and Department of Chemistry, University of Aveiro, Aveiro, Portugal; ISEIT/Viseu-Instituto Piaget, Estrada do Alto do Gaio, Galifonge, Lordosa, Viseu, Portugal.

Advances in Clinical Chemistry

|March 16, 2016

Summary

Immunosensors offer rapid, accurate detection of cardiac and cancer biomarkers, as well as pathogens. Nanotechnology enhances these biosensors for improved clinical diagnostics and point-of-care applications.

Keywords:

Biomarkers Clinical diagnostics Electrochemical Hormones Immunosensor Optical Piezoelectric Point-of-care Proteins Virus

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Area of Science:

Biomedical Engineering
Nanotechnology
Clinical Diagnostics

Background:

Biosensors are crucial for detecting diseases due to their specificity and use with real samples.
Nanotechnology integration into immunosensors significantly enhances sensitivity through antibody-antigen interactions.
Current diagnostic technologies aim for cost-effectiveness, speed, and accuracy.

Purpose of the Study:

To review various immunosensor types and their clinical diagnostic applications.
To highlight the role of immunosensors in point-of-care testing over the last six years.
To evaluate future perspectives of immunosensors in clinical diagnostics.

Main Methods:

Review of recent literature (6 years) on immunosensor technology.
Categorization of immunosensors based on detection principles and applications.
Analysis of performance metrics for clinical biomarker detection.

Main Results:

Immunosensors demonstrate high sensitivity and specificity for cardiac and cancer biomarkers.
Applications extend to detecting hormones, pathogenic bacteria, and viruses.
Point-of-care applications are rapidly advancing.

Conclusions:

Immunosensors are effective tools for rapid and accurate clinical diagnostics.
Nanotechnology-enhanced immunosensors show great promise for future healthcare.
Further development is expected to expand their role in point-of-care testing.