Related Experiment Video
Updated: Mar 24, 2026
Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
Published on: April 19, 2021
[Optimization of the immunohistochemical diagnosis of AL amyloidosis using novel antibodies]
L M Mikhaleva1, Z V Gioeva1, K Rëken2
1Research Institute of Human Morphology, Moscow, Russia.
Objective:
to improve the immunohistochemical diagnosis of AL amyloidosis, by generating novel peptide antibodies against the variable and constant regions of the κ-light chains.
Material And Methods:
All amyloidogenic κ-light chains were sought in the scientific literature and the database of the National Center for Biotechnology Information. On the basis of the findings, a chain was formed from the most common amino acid residues that were used to choose peptide regions for immunization. Four antibodies were generalized via immunization of rabbits with two peptides that corresponded to the variant or constant regions of κ-light chains.
Results:
The specificity of the obtained antibodies was confirmed using a series of 222 biopsy specimens from 193 patients with AL, AA, transthyretin, or insulin amyloidosis. All the novel polyclonal peptide antibodies produced positive staining in cases of ALκ amyloidosis.
Conclusion:
The generated polyclonal peptide antibodies against the variable and constant regions of κ-light chains are able to improve the immunohistochemical diagnosis of amyloidosis.
More Related Videos
10:04Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
Published on: October 20, 2017
13:31Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis
Published on: February 12, 2015