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Updated: Mar 24, 2026
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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
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Enhanced fluorescent resonant energy transfer of DNA conjugates complexed with surfactants and divalent metal ions
Taeseok Oh1, Jae-Young Choi, Michael J Heller
1Materials Science and Engineering, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA.
The Analyst
|March 18, 2016
Abstract:
Dimerization and resultant quenching of donor and acceptor dyes conjugated on DNA causes loss of fluorescent resonant energy transfer (FRET) efficiency. However, when complexed with surfactants and divalent metal ions, sheathing effects insulate and shield the DNA structures, reducing dimerization and quenching which leads to significant enhancement of FRET efficiency.
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