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Related Experiment Video

Updated: Mar 24, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system.

E Bertin1, M Piccoli1, C Franzin1

  • 1Stem Cells and Regenerative Medicine Laboratory, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padova, Italy.

Stem Cell Research
|March 19, 2016
PubMed
Summary

Researchers generated induced pluripotent stem (iPS) cells from mouse amniotic fluid (AF) cells using a non-viral PiggyBac transposon system. These iPS cells demonstrated pluripotency, differentiating into all three germ layers in vitro and in vivo.

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Area of Science:

  • Stem cell biology
  • Reproductive biology
  • Genetics

Background:

  • Induced pluripotent stem (iPS) cells are crucial for regenerative medicine and disease modeling.
  • Generating iPS cells typically involves viral methods, posing potential risks.
  • Amniotic fluid (AF) is an accessible source for cell collection during prenatal diagnosis.

Purpose of the Study:

  • To generate induced pluripotent stem (iPS) cells from mouse amniotic fluid (AF) cells.
  • To utilize a non-viral, PiggyBac (PB) transposon-based system for iPS cell generation.
  • To assess the pluripotency and differentiation capacity of the derived iPS cells.

Main Methods:

  • Isolation of mouse amniotic fluid (AF) cells.
  • Transfection of AF cells with the PiggyBac (PB) transposon system carrying reprogramming factors.
  • Culture and selection of induced pluripotent stem (iPS) cell colonies.
  • In vitro and in vivo differentiation assays to confirm pluripotency.

Main Results:

  • Successfully generated multiple induced pluripotent stem (iPS) cell lines from mouse AF cells.
  • The non-viral PiggyBac (PB) transposon system enabled efficient reprogramming.
  • All derived iPS cell lines exhibited characteristic markers of pluripotency.
  • Confirmed differentiation potential into derivatives of all three germ layers (ectoderm, mesoderm, endoderm) in vitro and in vivo.

Conclusions:

  • Mouse amniotic fluid (AF) is a viable and accessible source for generating induced pluripotent stem (iPS) cells.
  • The PiggyBac (PB) transposon system offers a safe and effective non-viral method for iPS cell generation from AF cells.
  • The generated iPS cells possess stable pluripotency, making them valuable for research and potential therapeutic applications.