Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

100.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.8K
Sanger Sequencing01:57

Sanger Sequencing

778.0K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
778.0K
Genomics02:02

Genomics

41.7K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
41.7K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.5K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.5K
RNA-seq03:21

RNA-seq

12.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sengstaken-Blakemore Tube Placement: A Simulation-Based Training Program for a High-Acuity, Low-Frequency Procedure.

MedEdPORTAL : the journal of teaching and learning resources·2026
Same author

Convergent and lineage-specific genomic changes shape adaptations in sugar-consuming birds.

Science (New York, N.Y.)·2026
Same author

Cell Lines CoCoPUTs: A Database of Codon and Codon-pair Usage Frequencies in Cell Lines.

Journal of molecular biology·2026
Same author

Comparative population genomics reveals convergent adaptation across independent origins of avian obligate brood parasitism.

Nature ecology & evolution·2025
Same author

Building Pediatric Cancer Cohorts and Accessing Data Using Childhood Cancer Data Initiative Tools.

JCO clinical cancer informatics·2025
Same author

Genomic resources for comparative analyses of avian obligate brood parasitism.

The Journal of heredity·2025
Same journal

Development of CypriSSR: a genome-wide, chromosome-level microsatellite database for multiple cyprinidae species.

Database : the journal of biological databases and curation·2026
Same journal

KitBase Expanded: An Integrated Genomic and Phenotypic Resource for 3,268 Fast-Neutron-Irradiated Rice Mutants.

Database : the journal of biological databases and curation·2026
Same journal

PhaLP 2.0: extending the community-oriented phage lysin database with a SUBLYME pipeline for metagenomic discovery.

Database : the journal of biological databases and curation·2026
Same journal

A similarity metric, rubric, and unified hierarchy for biomedical publication types and study designs.

Database : the journal of biological databases and curation·2026
Same journal

GUTAID: a curated database linking gut microbial antigens to autoimmune mechanisms.

Database : the journal of biological databases and curation·2026
Same journal

Rosetta Statements: simplifying FAIR knowledge graph construction with a user-centred approach.

Database : the journal of biological databases and curation·2026
See all related articles

Related Experiment Video

Updated: Mar 24, 2026

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

13.9K

High-performance integrated virtual environment (HIVE): a robust infrastructure for next-generation sequence data

Vahan Simonyan1, Konstantin Chumakov2, Hayley Dingerdissen3

  • 1Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, MD 20993, USA vahan.simonyan@fda.hhs.gov vahansim@gmail.com.

Database : the Journal of Biological Databases and Curation
|March 19, 2016
PubMed
Summary
This summary is machine-generated.

The High-performance Integrated Virtual Environment (HIVE) offers a secure cloud platform for next-generation sequencing (NGS) data analysis. Its innovative data model and computation-to-data approach enhance efficiency and flexibility for researchers.

More Related Videos

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

7.6K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

35.0K

Related Experiment Videos

Last Updated: Mar 24, 2026

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

13.9K
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

7.6K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

35.0K

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Next-generation sequencing (NGS) generates vast amounts of data, requiring robust storage and computational solutions.
  • Existing distributed computing environments often face challenges with data management and scalability for NGS workloads.

Purpose of the Study:

  • To introduce the High-performance Integrated Virtual Environment (HIVE) as a novel cloud infrastructure for NGS data.
  • To describe HIVE's architecture, data model, and security features for efficient and secure data analysis.

Main Methods:

  • HIVE utilizes a cloud control server to virtualize services, abstracting computational requests from operating system processes.
  • A novel 'computation-to-data' paradigm minimizes data movement, reducing hardware and network strain.
  • The honeycomb data model integrates metadata using an object-oriented approach with a unified API for diverse data types.

Main Results:

  • HIVE provides secure web access for depositing, retrieving, annotating, and computing on NGS data.
  • The architecture ensures robustness and flexibility through service virtualization and an abstraction layer.
  • The honeycomb model simplifies data type integration and supports granular access control for enhanced security.

Conclusions:

  • HIVE offers an efficient and secure platform for NGS analysis, benefiting researchers and scientists.
  • The system's design facilitates streamlined data management and analysis, supporting the introduction of new data types.
  • HIVE is actively supported and welcomes collaborations for further development and application.