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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
A colorimetric and fluorescent dual probe for palladium in aqueous medium and live cell imaging
Jin-wu Yan1, Xiao-lin Wang, Qi-feng Tan
1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China.
Abstract:
A colorimetric and fluorescent dual probe for palladium species was rationally developed by combining the resorufin fluorophore with allyl chloroformate. The probe enables the visual detection of palladium based on its vivid color change from pale yellow to pink and its fluorescence off-on response to palladium in PBS solution. The detection limit was calculated to be as low as 2.1 nM. The live cell imaging results showed that this probe could be used as an effective fluorescent probe for detecting intracellular palladium species. All these results featured its promising application prospects in the palladium analytical field.
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