A review on electronic bio-sensing approaches based on non-antibody recognition elements

Area of Science:

• Biotechnology
• Nanotechnology
• Analytical Chemistry

Background:

• Antibody-based biosensors offer high selectivity and sensitivity for diagnostics.
• However, antibodies have limitations including fragility, large size, and complex preparation.
• These limitations impact shelf-life, stability, and reproducibility in biosensing applications.

Purpose of the Study:

• To review recent advances in electronic biosensing technologies.
• To highlight non-antibody recognition elements like functional liposomes, aptamers, and synthetic peptides.
• To focus on the progress of field-effect transistor (FET) sensing platforms utilizing these synthetic elements.

Main Methods:

• Comprehensive literature review of recent publications on electronic biosensors.
• Focus on biosensors employing synthetic recognition elements.
• Analysis of field-effect transistor (FET) sensing platforms.

Main Results:

• Significant progress in FET-based sensing platforms utilizing synthetic recognition elements.
• Synthetic elements demonstrate potential for enhanced stability, reproducibility, and simplified preparation compared to antibodies.
• Functional liposomes, aptamers, and synthetic peptides show promise as alternatives to antibodies.

Conclusions:

• Synthetic recognition elements offer a viable and advantageous alternative to antibodies in electronic biosensor development.
• Continued research in this area is crucial for advancing diagnostic and healthcare applications.
• FET-based biosensors with synthetic elements represent a rapidly growing field with substantial potential.