Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.6K
Nuclear Export01:42

Nuclear Export

5.2K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.2K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

4.0K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
4.0K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

13.3K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
13.3K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

9.0K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unconventional activation of the proto-oncogene FGFR1 by extracellular phosphate via H<sub>2</sub>O<sub>2</sub>-mediated kinase oxidation.

Cell reports·2026
Same author

Elevated GCN2 levels in cancer cells confer protection from mitotic stress and faster cell movement.

Cellular oncology (Dordrecht, Netherlands)·2026
Same author

isMap - immunological synapse map analysis program.

Frontiers in immunology·2026
Same author

LRRC59 cooperates with nuclear transporters to restrain the nuclear envelope repair machinery and safeguard genome integrity.

Nature communications·2025
Same author

Lysosomal membrane homeostasis and its importance in physiology and disease.

Nature reviews. Molecular cell biology·2025
Same author

Phosphoinositides as regulators of membrane contact sites.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2025
Same journal

Emerging role of organelles in cell migration.

Current opinion in cell biology·2026
Same journal

Nuclear adaptation in cell migration.

Current opinion in cell biology·2026
Same journal

Patterns in motion: Choreographing dynamic cell behaviours during tissue repair.

Current opinion in cell biology·2026
Same journal

Quo vadis reconstituted cell surfaces? Purpose and future perspectives for minimal systems of the cell plasma membrane.

Current opinion in cell biology·2026
Same journal

Nuclear determinants of mRNA and protein isoforms.

Current opinion in cell biology·2026
Same journal

Substrate selectivity as a paradigm shift in mTORC1 signaling.

Current opinion in cell biology·2026
See all related articles

Related Experiment Video

Updated: Mar 23, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.8K

Closing a gap in the nuclear envelope.

Marina Vietri1, Harald Stenmark2, Coen Campsteijn1

  • 1Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N-0379 Oslo, Norway; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, N-0379 Oslo, Norway.

Current Opinion in Cell Biology
|March 27, 2016
PubMed
Summary
This summary is machine-generated.

The nuclear envelope reforms after mitosis, requiring endoplasmic reticulum membrane remodeling and nuclear pore complex assembly. The endosomal sorting complex (ESCRT) machinery plays a key role in these crucial cell division events.

More Related Videos

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.9K
Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
06:23

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

Published on: January 17, 2025

1.3K

Related Experiment Videos

Last Updated: Mar 23, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.8K
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.9K
Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
06:23

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

Published on: January 17, 2025

1.3K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The nuclear envelope (NE) maintains cell compartmentalization, regulating molecular traffic via nuclear pore complexes (NPCs).
  • NE breakdown during mitosis necessitates its reformation for cell cycle progression.

Purpose of the Study:

  • To review recent advancements in understanding NE reformation and NPC biogenesis.
  • To highlight the role of the endosomal sorting complex required for transport (ESCRT) machinery in NE and NPC formation.

Main Methods:

  • Literature review of recent studies on nuclear envelope and nuclear pore complex formation.
  • Focus on research implicating membrane remodeling machinery in these processes.

Main Results:

  • NE reformation involves dynamic endoplasmic reticulum (ER) membrane remodeling and NPC assembly.
  • NPC deposition continues into interphase after initial NE reestablishment.
  • The ESCRT machinery is implicated in NE reformation and NPC generation.

Conclusions:

  • The ESCRT pathway is a critical regulator of NE and NPC biogenesis.
  • Understanding these processes is vital for comprehending cell division and nuclear organization.