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Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis
A G Cherednichenko1, M A Dymova2,3, O A Solodilova4
1Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia.
Abstract:
Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.
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