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Updated: Mar 23, 2026
Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
Published on: April 6, 2021
[THE DNA DIAGNOSTIC OF PSEUDOMONAS INFECTION]
Abstract:
The modern laboratory diagnostic of pseudomonas infection, including bacteriologic analysis with subsequent identication of agent, is time-consuming and it is not every time effective because of modifiability of characteristics of the mentioned agent. The article presents the laboratory mode of identification of Pseudomonas aeruginosa based on electrophoresis analysis of DNA isolatedfrom wound samples or microbial mass grew on solid nutritional medium. The wound sample or pure growth is placed into physiological solution and is warmed under 990C. This action results in DNA releasing into supernatant which sample is underwent to standard horizontal electrophoresis in agarose gel with ethidium bromide. The presence on electrophoregrams of three bands of DNA fluorescent in ultraviolet with size of fragments about 10,000, 6000-8000a and less than 750 pairs of nucleotides testifies the presence of P. aeruginosa in the analyzed biomaterial. The given mode permits to identify both chromogenic and pigment-free strains of P. aeruginosa and to differentiate them of other representatives of non-fermentative gram-negative bacteria and most significant genera and species of family of Enterobacteriaceae. The identification of P. aeruginosa in wound sample using the proposed mode implementing without its bacteriological analysis, can be used as technique of express diagnostic of pseudomonas infection.
Insights
This study presents a rapid DNA electrophoresis method for identifying Pseudomonas aeruginosa infections. The technique quickly detects the bacteria in wound samples, improving diagnostic speed and effectiveness.
Area of Science:
- Clinical Microbiology
- Molecular Diagnostics
- Bacteriology
Background:
- Traditional Pseudomonas aeruginosa diagnostics are slow and can be unreliable due to bacterial adaptability.
- Accurate and rapid identification of Pseudomonas aeruginosa is crucial for effective pseudomonas infection treatment.
Purpose of the Study:
- To develop and validate a novel laboratory method for the rapid identification of Pseudomonas aeruginosa.
- To offer an alternative to conventional bacteriologic analysis for diagnosing pseudomonas infections.
Main Methods:
- DNA was isolated from wound samples or microbial cultures and heated to release DNA.
- Standard horizontal electrophoresis in agarose gel with ethidium bromide was performed.
- Electrophoresis patterns were analyzed for specific DNA fragment sizes indicating Pseudomonas aeruginosa presence.
Main Results:
- The presence of three distinct DNA bands (approx. 10,000 bp, 6000–8000 bp, and <750 bp) on electrophoregrams confirmed Pseudomonas aeruginosa.
- This method successfully identified both pigment-producing and non-pigment-producing strains.
- It differentiated Pseudomonas aeruginosa from other Gram-negative bacteria, including Enterobacteriaceae.
Conclusions:
- The DNA electrophoresis method provides a fast and effective means for identifying Pseudomonas aeruginosa directly from wound samples.
- This technique bypasses the need for traditional bacteriological analysis, serving as an express diagnostic tool for pseudomonas infections.
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