Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.2K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

975
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
975
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.9K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.1K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pupil aberration coefficients in plane-symmetric optical systems.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same author

Single-step alignment of a two-mirror telescope using sigma vectors in nodal aberration theory.

Applied optics·2026
Same author

Two-level optimizer for large-scale metasurfaces with strong near-field coupling.

Optics express·2026
Same author

Wide-range High-precision Eye-tracking based on Purkinje Reflections.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same author

Analytical aberration theory for plane-symmetric optical systems and its application in distortion analysis of spectrometers: erratum.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same author

Source wavefront generation for a non-interferometric reconfigurable null test using a photonic lantern.

Optics letters·2025

Related Experiment Video

Updated: Mar 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

9.1K

Real Time Gabor-Domain Optical Coherence Microscopy for 3D Imaging.

Jannick P Rolland1, Cristina Canavesi2, Patrice Tankam1

  • 1University of Rochester, Rochester, NY, USA 14627.

Studies in Health Technology and Informatics
|April 6, 2016
PubMed
Summary

This study introduces a novel Gabor-domain optical coherence microscopy (GD-OCM) system for fast, nondestructive 3D tissue imaging. The technology achieves high-resolution volumetric scans, paving the way for clinical integration.

More Related Videos

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

7.4K
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
06:19

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Published on: November 11, 2017

11.3K

Related Experiment Videos

Last Updated: Mar 23, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

9.1K
Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

7.4K
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
06:19

In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Published on: November 11, 2017

11.3K

Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Microelectromechanical Systems

Background:

  • Need for fast, robust, nondestructive 3D imaging in clinical applications.
  • Limitations of current microscopic tissue characterization techniques.

Purpose of the Study:

  • Develop a high-definition, fast, and robust 3D imaging system for microscopic tissue characterization.
  • Enable integration of advanced imaging technology into clinical workflows.

Main Methods:

  • Custom microelectromechanical system (MEMS)-based 2D scanner for 55 kHz A-scan acquisition.
  • Multi-level GPU architecture for accelerated data processing.
  • Bio-inspired liquid lens for dynamic depth of focusing and wide field of view.

Main Results:

  • Achieved high-definition micrometer-class volumetric images using Gabor-domain optical coherence microscopy (GD-OCM).
  • Demonstrated invariant 2 μm resolution across a 1 × 1 × 0.6 mm³ imaging volume.
  • Acquired volumetric scans in under 2 minutes, including in vivo human skin and excised human cornea.

Conclusions:

  • The developed GD-OCM system offers fast, high-resolution, nondestructive 3D imaging capabilities.
  • The bio-inspired dynamic focusing enhances imaging quality over a large field of view.
  • This technology is poised for integration into clinical environments for improved tissue characterization.