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Updated: Mar 23, 2026

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Circular RNAs in Eukaryotic Cells.

Liang Chen¹, Chuan Huang¹, Xiaolin Wang¹

¹School of Life Sciences & CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China, Hefei, Anhui Province 230027, P.R. China.

Current Genomics

|April 6, 2016

Summary

This summary is machine-generated.

Circular RNAs (circRNAs) are abundant eukaryotic transcripts. These regulatory elements, once thought to be noise, are increasingly linked to human diseases and may be classified into subtypes.

Keywords:

Biogenesis EIciRNA Transcription.circRNA microRNA microRNA sponge ncRNA

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Identification of Circular RNAs using RNA Sequencing

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

Circular RNAs (circRNAs) are a significant class of transcripts in eukaryotic cells, identified across various model organisms and humans.
The discovery of circRNAs has substantially increased the understanding of transcriptome complexity.
Previously considered transcriptional noise, circRNAs are now recognized as potential genome-coded regulatory elements.

Purpose of the Study:

To summarize current knowledge regarding the biogenesis and physiological functions of circRNAs.
To discuss the potential involvement of circRNAs in human diseases.
To propose a classification of circRNAs into subtypes based on their features, such as cytoplasmic circRNAs, nuclear circRNAs, and exon-intron circRNAs (EIciRNAs).

Main Methods:

Literature review and synthesis of existing research on circRNA biogenesis, function, and disease association.
Analysis of sequence composition and cellular localization data to support proposed circRNA subtypes.
Comparative analysis across different species to understand the prevalence and conservation of circRNAs.

Main Results:

Thousands of circRNAs, primarily formed via exon back-splicing, have been identified.
Substantial knowledge has been gained regarding circRNA biogenesis and physiological roles.
Evidence suggests circRNAs are implicated in certain human diseases and exhibit diverse features, supporting subtype classification.

Conclusions:

Circular RNAs are important regulatory molecules, not merely transcriptional byproducts.
The proposed classification into cytoplasmic, nuclear, and EIciRNAs aids in understanding their diverse roles.
The field of circRNA research is rapidly evolving, with many aspects still requiring exploration.