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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

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Published on: April 14, 2015

Multiplexed Sequence Encoding: A Framework for DNA Communication.

Bijan Zakeri^1,2, Peter A Carr^2,3, Timothy K Lu^1,2

¹Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Research Laboratory of Electronics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States of America.

|April 7, 2016

Summary

This summary is machine-generated.

Synthetic DNA enables new communication methods by fragmenting short messages across multiple DNA molecules. Novel tools for DNA encoding, secret sharing, and data extraction advance DNA-based information transfer.

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Area of Science:

Biotechnology
Bioinformatics
Synthetic Biology

Background:

Synthetic DNA offers efficient and stable non-biological information storage.
Advancements in DNA synthesis and sequencing technologies are expanding applications in data storage and communication.
Traditional DNA communication focuses on transferring complete information sets.

Purpose of the Study:

To explore DNA's potential for communicating short messages fragmented across multiple DNA molecules.
To develop novel tools for DNA-based communication, addressing encoding, transfer, and extraction.
To enable more widespread information communication via DNA.

Main Methods:

Developed DNA-based individualized keyboards (iKeys) for plaintext to DNA conversion, minimizing homopolymers.
Implemented a secret-sharing system, Multiplexed Sequence Encoding (MuSE), for message concealment across DNA molecules.
Achieved chromatogram patterning through multiplexed sequencing for novel data extraction.

Main Results:

Successfully converted plaintext to DNA using iKeys, enhancing synthesis and sequencing.
Demonstrated secure message fragmentation and reconstruction using the MuSE system.
Established a new data extraction method via multiplexed sequencing and chromatogram patterning.

Conclusions:

Novel tools (iKeys, MuSE) facilitate fragmented DNA communication.
Chromatogram patterning offers a new approach for DNA data extraction.
These advancements pave the way for broader adoption of DNA for information communication.