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Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Updated: Mar 23, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Nuclear m(6)A Reader YTHDC1 Regulates mRNA Splicing.

Ian A Roundtree¹, Chuan He²

¹Department of Biochemistry and Molecular Biology, Medical Scientist Training Program, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.

Trends in Genetics : TIG

|April 7, 2016

Summary

This summary is machine-generated.

N(6)-Methyladenosine (m(6)A) impacts genetic information flow. The nuclear m(6)A reader YTHDC1 protein influences mRNA splicing, affecting gene expression across the transcriptome.

Keywords:

N(6)-methyladenosine YTHDC1 mRNA splicing

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Published on: June 30, 2022

Area of Science:

Epigenetics
Molecular Biology
RNA Biology

Background:

N(6)-Methyladenosine (m(6)A) is a prevalent epitranscriptomic modification.
m(6)A influences diverse cellular processes, including gene expression and RNA metabolism.
Nuclear m(6)A reader proteins play crucial roles in mediating m(6)A functions.

Purpose of the Study:

To investigate the role of the nuclear m(6)A reader protein YTHDC1 in mRNA splicing.
To provide a transcriptome-wide analysis of splicing alterations regulated by YTHDC1.

Main Methods:

RNA sequencing to profile splicing changes.
Bioinformatic analysis to identify affected splicing events.
m(6)A-seq or related techniques to assess m(6)A modification patterns.

Main Results:

YTHDC1 was identified as a key regulator of mRNA splicing.
A significant number of transcriptome-wide splicing events were found to be affected by YTHDC1.
Specific splicing patterns influenced by YTHDC1 were characterized.

Conclusions:

The nuclear m(6)A reader YTHDC1 plays a significant role in post-transcriptional gene regulation via mRNA splicing.
Understanding YTHDC1's function provides insights into the epitranscriptome's impact on gene expression.
This study highlights the importance of m(6)A modifications in regulating splicing dynamics.