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Updated: Mar 23, 2026
Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
Published on: July 6, 2019
[NaHS inhibits A549 cell injury induced by lipopolysaccharide]
Zhancong Niu1, Shengjun Yang2, Guochen Duan3
1Department of Infectious Diseases, Hebei Provincial People's Hospital, Shijiazhuang 050051, China.
Objective:
To observe the effect of NaHS on lipopolysaccharide (LPS)-induced injury of A549 lung cancer cells and explore the possible mechanisms.
Methods:
A549 cells were divided into 4 groups: control group, 10 μg/mL LPS treated group, 10 μmol/L NaHS treated group and the group treated with 10 μg/mL LPS plus 10 μmol/L NaHS. After treatment for 24 hours, C-reactive protein (CRP) content in culture solution was measured by ELISA, and the cell viability was detected by MTT assay and lactate dehydrogenase (LDH) assay. Transepithelial electrical resistance (TER) was evaluated by EVOM resistance meter, and the expression and distribution of zonula occludens-1 (ZO-1) protein was examined by immunofluorescence cytochemistry.
Results:
There were no significant differences in CRP content, TER, cell viability, the expression and distribution of ZO-1 between the control group and the NaHS group. Compared with the control group, CRP content distinctly increased, TER, cell viability and ZO-1 expression markedly decreased, and ZO-1 distribution was disrupted in the LPS group. Compared with the LPS group, CRP level was distinctly reduced, TER, cell viability and ZO-1 expression were raised, and the disrupted ZO-1 distribution was partly reversed in the LPS plus NaHS treated group.
Conclusion:
NaHS can inhibit A549 cell injury induced by LPS via increasing cell viability, TER and the expression of ZO-1 as well as decreasing CRP level.
