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Proteome-wide dataset supporting functional study of tyrosine kinases in breast cancer

Nicos Angelopoulos1, Justin Stebbing1, Yichen Xu1

  • 1Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 ONN, UK.

Data in Brief
|April 8, 2016
PubMed

Insights

This study reveals the global functional proteomic landscape of tyrosine kinases (TKs) in breast cancer using RNAi and SILAC proteomics. The data, now publicly available, offers a valuable resource for cancer research and understanding TK signaling networks.

Area of Science:

  • Biochemistry
  • Proteomics
  • Cancer Biology

Background:

  • Tyrosine kinases (TKs) are crucial for cellular functions.
  • Dysregulated TK signaling is implicated in cancer development.
  • Many TKs remain understudied, limiting our understanding of their roles.

Purpose of the Study:

  • To globally map the functional proteomic landscape of TKs in breast cancer.
  • To identify the integrated signaling network regulated by TKs.
  • To create an accessible resource for the scientific community.

Main Methods:

  • RNA interference (RNAi) for gene silencing.
  • Stable Isotope Labeling with Amino acids in cell culture (SILAC) for quantitative proteomics.
  • High-throughput proteomic analysis to profile TK activity.

Main Results:

  • A comprehensive proteomic landscape of TKs in breast cancer was elucidated.
  • A highly integrated signaling network regulated by TKs was highlighted.
  • The study identified previously underappreciated TK functions in cancer.

Conclusions:

  • The proteomic data provides a valuable resource for cancer research.
  • Understanding TK signaling networks is key to developing targeted cancer therapies.
  • Open access data facilitates further investigation into TKs in oncogenesis.
Keywords:
Breast cancerCell signalingProteomicsSILACTyrosine kinases

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