Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

4.2K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
4.2K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

9.8K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.8K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

19.3K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
19.3K
Endoplasmic Reticulum01:39

Endoplasmic Reticulum

118.2K
The Endoplasmic Reticulum (ER) in eukaryotic cells is a substantial network of interconnected membranes with diverse functions, from calcium storage to biomolecule synthesis. A primary component of the endomembrane system, the ER manufactures phospholipids critical for membrane function throughout the cell. Additionally, the two distinct regions of the ER specialize in the manufacture of specific lipids and proteins.
118.2K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

4.4K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
4.4K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.2K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A new probe illuminates endo-lysosomal Ca2+ measurements: A role for vesicular IP3 receptors?

The Journal of cell biology·2025
Same author

TPC2 gating: Trying to break the enigma code.

Cell calcium·2024
Same author

Optical profiling of autonomous Ca<sup>2+</sup> nanodomains generated by lysosomal TPC2 and TRPML1.

Cell calcium·2023
Same author

Two-pore channels: going with the flows.

Biochemical Society transactions·2022
Same author

NAADP-Mediated Ca<sup>2+</sup> Signalling.

Handbook of experimental pharmacology·2022
Same author

Acidic Ca<sup>2+</sup> stores and immune-cell function.

Cell calcium·2021
Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026
Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026
Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026
Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026
Same journal

Implications of Rho GTPase signaling in cancer immunotherapy.

Biochemical Society transactions·2026
See all related articles

Related Experiment Video

Updated: Mar 22, 2026

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.4K

Ca2+ dialogue between acidic vesicles and ER.

Anthony J Morgan1

  • 1Department of Pharmacology, University of Oxford, Mansfield Road, Oxford OX1 3QT, U.K. Anthony.morgan@pharm.ox.ac.uk.

Biochemical Society Transactions
|April 13, 2016
PubMed
Summary
This summary is machine-generated.

Cellular calcium (Ca2+) signaling involves the endoplasmic reticulum (ER) and acidic stores. This review explores their interaction in generating Ca2+ signals, focusing on inositol 1,4,5-trisphosphate (IP3) and nicotinic acid adenine dinucleotide phosphate (NAADP) pathways.

Keywords:
Ca2+IP3LysosomeNAADPTPCendoplasmic reticulum

More Related Videos

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

19.6K
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
13:40

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

Published on: July 7, 2011

18.8K

Related Experiment Videos

Last Updated: Mar 22, 2026

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.4K
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED
09:32

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading TED

Published on: May 7, 2013

19.6K
Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
13:40

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

Published on: July 7, 2011

18.8K

Area of Science:

  • Cellular Biology
  • Molecular Signaling
  • Physiology

Background:

  • Extracellular stimuli trigger intracellular second messengers, modulating Ca2+ release from organelles via Ca2+-channel complexes.
  • The endoplasmic reticulum (ER) pathway involves inositol 1,4,5-trisphosphate (IP3) and IP3 receptors (IP3R).
  • A parallel pathway utilizes nicotinic acid adenine dinucleotide phosphate (NAADP) to release Ca2+ from acidic stores, dependent on two-pore channels (TPCs).

Purpose of the Study:

  • To review the physical and functional interactions between ER and acidic Ca2+ stores.
  • To elucidate the generation and shaping of global and local Ca2+ signals by these organelles.
  • To emphasize the bidirectional communication between ER and acidic Ca2+ stores in cellular signaling.

Main Methods:

  • Literature review and synthesis of existing research on Ca2+ signaling pathways.
  • Analysis of the roles of IP3R and TPCs in Ca2+ release.
  • Examination of the interplay between ER and acidic Ca2+ stores.

Main Results:

  • ER and acidic Ca2+ stores interact dynamically to control Ca2+ signaling.
  • NAADP-mediated Ca2+ release from acidic stores is crucial and TPC-dependent.
  • The cross-talk between these organelles is essential for complex Ca2+ signal dynamics.

Conclusions:

  • Understanding the dialogue between ER and acidic Ca2+ stores is key to deciphering cellular Ca2+ signaling.
  • This interaction influences both local and global Ca2+ dynamics.
  • Further research into TPCs and NAADP signaling promises new insights into cellular regulation.