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Covalent Labeling with Diethylpyrocarbonate for Studying Protein Higher-Order Structure by Mass Spectrometry
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Revealing Higher Order Protein Structure Using Mass Spectrometry.
Brian T Chait1, Martine Cadene2, Paul Dominic Olinares3
1Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA. chait@rockefeller.edu.
Journal of the American Society for Mass Spectrometry
|April 16, 2016
Summary
Mass spectrometry (MS) is revolutionizing structural biology by enabling precise measurement of proteins and complexes. This perspective highlights MS techniques for determining higher-order protein structures and advancing molecular imaging.
Area of Science:
- Structural biology
- Biochemistry
- Analytical chemistry
Background:
- Mass spectrometry (MS) offers rapid, sensitive, and accurate measurement capabilities for peptides, proteins, and protein assemblies.
- These advancements position MS as a critical tool in modern structural biology research.
Purpose of the Study:
- To provide a perspective on the expanding role of mass spectrometric techniques in elucidating higher-order protein structures.
- To discuss the utility of MS in various structural biology applications.
Main Methods:
- Mass spectrometry (MS) for high-resolution structural analysis.
- MS for compositional analysis of endogenous protein complexes.
- MS for stoichiometry determination and integrated structural elucidation approaches.
Main Results:
- MS serves as an enabling tool for high-resolution structural biologists.
- MS facilitates compositional analysis and stoichiometry determination of protein complexes.
- Integrated MS approaches are effective for structural elucidation of protein complexes.
Conclusions:
- Mass spectrometry is increasingly vital for understanding protein structures and complexes.
- Future MS-based techniques promise to drive the development of multi-scale molecular microscopy.
Keywords:
Electrospray ionizationEndogenous protein complexesFolding state in solutionHigher order structureMALDIMass spectrometryMolecular microscopeNative mass spectrometryProtein assemblyProtein complexProtein compositionStoichiometryStructural biologyX-ray crystallographyMore Related Videos
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