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Lamin B methylation and assembly into the nuclear envelope.

D Chelsky1, C Sobotka, C L O'Neill

  • 1Central Research and Development Department, E. I. du Pont de Nemours & Company, Wilmington, Delaware 19898.

The Journal of Biological Chemistry
|May 5, 1989
PubMed
Summary

Methylation of lamin B is crucial for nuclear envelope assembly during the cell cycle. Inhibiting methylation with MTA delays this process, showing it

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Lamin B undergoes reversible methylation and phosphorylation during the mammalian cell cycle.
  • Understanding the role of lamin B methylation in nuclear envelope formation is essential for cell cycle research.

Purpose of the Study:

  • To investigate the functional role of lamin B methylation in nuclear envelope assembly.
  • To determine if methylation precedes or follows nuclear envelope formation.
  • To explore the relationship between lamin B methylation, dephosphorylation, and nuclear envelope assembly.

Main Methods:

  • Isolation of mitotic cells using nocodazole.
  • Treatment with methylthioadenosine (MTA) to inhibit methylation.
  • Monitoring lamin B methylation, dephosphorylation, and nuclear envelope assembly.
  • Utilizing Chinese hamster ovary cells and PC 13 stem cells.

Main Results:

  • Lamin B methylation precedes nuclear envelope assembly as cells exit mitosis.
  • Inhibition of methylation with MTA delays both lamin B assembly and chromatin decondensation.
  • Dephosphorylation of lamin B can occur independently of assembly, indicating it's not sufficient for the process.
  • MTA's specific effect on methylation suggests it acts downstream of dephosphorylation.

Conclusions:

  • Lamin B methylation is a critical, rate-limiting step for nuclear envelope assembly.
  • Nuclear envelope formation requires coordinated events, including methylation and interactions between lamins.
  • Dephosphorylation alone is insufficient for nuclear envelope assembly; methylation must occur first.

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