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Updated: Mar 22, 2026
Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Published on: September 28, 2012
Cancer cell-targeted two-photon fluorescence probe for the real-time ratiometric imaging of DNA damage
Hua Zhang1, Kui Wang, Xiaopeng Xuan
1Henan Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Key Laboratory of Green Chemical Media and Reactions, Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, School of Chemistry and Chemical Engineering Institution, Henan Normal University, 453007 Xinxiang, China. zhh1106@htu.edu.cn zhanghua1106@163.com ghm@htu.edu.cn.
Abstract:
Real-time imaging of DNA damage in cancer cells could provide valuable information on the formation and development of cancer. Herein, a two-photon fluorescence probe was discovered. Through sequential ICT processes, it allows successful in vivo visualization of DNA damage in cancer cells by one/two-photon microscopic imaging or by the unaided eye and a hand-held ultraviolet lamp.
Insights
A novel two-photon fluorescence probe enables real-time imaging of DNA damage in cancer cells. This breakthrough allows for in vivo visualization, aiding cancer research and diagnostics.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Cancer Research
Background:
- Real-time imaging of DNA damage is crucial for understanding cancer development.
- Current methods may have limitations in sensitivity or real-time application.
Purpose of the Study:
- To develop a novel fluorescence probe for visualizing DNA damage in cancer cells.
- To enable real-time, in vivo imaging of DNA damage using advanced microscopy techniques.
Main Methods:
- Discovery and synthesis of a two-photon fluorescence probe.
- Utilizing sequential intramolecular charge transfer (ICT) processes within the probe.
- Employing one/two-photon microscopic imaging for in vivo visualization.
- Assessing visualization by the unaided eye under ultraviolet (UV) lamp illumination.
Main Results:
- The developed probe successfully visualized DNA damage in cancer cells in vivo.
- The probe demonstrated efficacy with both advanced microscopic imaging and simple UV lamp visualization.
- Sequential ICT processes were key to the probe's imaging capabilities.
Conclusions:
- The novel two-photon fluorescence probe offers a powerful tool for real-time DNA damage detection in cancer.
- This technology has potential applications in cancer diagnostics and therapeutic monitoring.
- The probe's dual-mode visualization enhances its versatility and accessibility.
