Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calibration methods for quantitative image processing in electron microscopy.

M K Lamvik1, S Davilla

  • 1Department of Anatomy, Duke University Medical Center, Durham, North Carolina 27710.

Journal of Electron Microscopy Technique
|February 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temperature directly affects the rate of irradiation-induced mass loss from phosphatidylcholine multilayers.

Ultramicroscopy·1991
Same author

Correction for specimen movement after acquisition of element-specific electron microprobe images.

Journal of microscopy·1989
Same author

Substrate properties affect the mass loss rate in collodion at liquid helium temperature.

Ultramicroscopy·1989
Same author

Dynamic hydration effects in an electron microscope cold stage.

Journal of electron microscopy technique·1988
Same author

Simple multi-process data sharing with the VAX/VMS operation system.

Journal of electron microscopy technique·1988
Same author

Real-time graphics display of mass variation or elemental concentration during electron beam microanalysis using a general purpose computer.

Journal of microscopy·1988
Same journal

Detection and identification of viruses by electron microscopy.

Journal of electron microscopy technique·2020
Same journal

A Simple Method for Quick-Freezing.

Journal of electron microscopy technique·2015
Same journal

Shrinkage in tertiary butanol prior to freeze drying.

Journal of electron microscopy technique·1991
Same journal

An alkali digestion method to expose connective tissue fibers: a scanning electron microscopy study of rat lung.

Journal of electron microscopy technique·1991
Same journal

Cross-sectional transmission electron microscopy of X-ray multilayer thin film structures.

Journal of electron microscopy technique·1991
Same journal

Dry fracturing and cutting techniques for scanning electron microscopy of poly-vinyl chloride particles: application to internal structure observations.

Journal of electron microscopy technique·1991
See all related articles

This study presents a digital image processing method to accurately determine the mass of microscopic particles using electron microscopy. This technique enhances quantitative analysis of electron micrographs for material science applications.

Area of Science:

  • Materials Science
  • Microscopy
  • Image Analysis

Background:

  • Digital images represent objects as numerical matrices.
  • Linear relationships between image data and physical properties allow for quantitative analysis.
  • Electron microscopy is a powerful tool for visualizing microscopic structures.

Purpose of the Study:

  • To develop a digital image processing method for determining microscopic particle mass.
  • To establish linear conditions for quantitative analysis of electron micrographs.
  • To calibrate the relationship between image transmission and mass thickness.

Main Methods:

  • Utilizing photographic densitometry of electron microscope films.
  • Applying quantitative image processing to establish linear relationships.

Related Experiment Videos

  • Employing digital computer methods for image display and integration.
  • Using polystyrene spheres as calibration standards.

    • Main Results:

    • Demonstrated a digital method to establish linear conditions for quantitative analysis.
    • Showcased the ability to determine particle mass from electron micrographs.
    • Generated calibration curves for non-linear transmission-to-mass thickness relationships.
    • Validated the method using polystyrene spheres.

    Conclusions:

    • The developed digital image processing technique enables accurate mass determination of microscopic particles.
    • This method enhances quantitative analysis in electron microscopy.
    • The approach is adaptable for various materials and calibration needs.