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Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Chromatographic Resolution01:15

Chromatographic Resolution

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In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
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Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
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Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
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Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
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Biennial Review of Planar Chromatography: 2013-2015.

Joseph Sherma1

  • 1Lafayette College, Department of Chemistry, Easton, PA 18042, USA.

Journal of AOAC International
|April 23, 2016
PubMed
Summary

This review covers key advances in planar chromatography from 2013-2015, including techniques, detection, and applications. It highlights progress in thin-layer chromatography (TLC) and preparative layer chromatography.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Planar chromatography encompasses techniques like thin-layer chromatography (TLC).
  • Continuous advancements are crucial for effective separation and analysis.

Purpose of the Study:

  • To review significant developments in planar chromatography.
  • To cover the period from November 1, 2013, to November 1, 2015.

Main Methods:

  • Literature review of scientific publications.
  • Categorization of advances into key areas of planar chromatography.

Main Results:

  • Comprehensive overview of apparatus and techniques for sample preparation and TLC separations.
  • Advances in detection, identification, and quantitative analysis of separated zones.

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  • Developments in preparative layer chromatography and thin-layer radiochromatography.

    • Conclusions:

    • The field of planar chromatography has seen notable progress in separation efficiency and analytical capabilities.
    • The review provides a valuable resource for understanding recent trends and applications in planar chromatography.