Programmed Cell Death Ligand 1 Expression in Canine Cancer
Kazuha Shosu1, Masashi Sakurai2, Kumi Inoue1
1Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yoshida, Yamaguchi, Japan.
In Vivo (Athens, Greece)
|April 24, 2016
Summary
Programmed cell death-ligand 1 (PD-L1) is expressed within most canine tumor cell lines and frequently in specific tumor types like melanoma and lymphoma. This finding identifies potential canine cancers for PD-1/PD-L1 antibody therapies.
Area of Science:
- Veterinary Oncology
- Immunology
- Cancer Research
Background:
- Antibody therapy targeting programmed cell death-1 (PD-1) and programmed cell death-ligand 1 (PD-L1) shows promise in human cancer treatment.
- Limited data exists regarding PD-L1 expression in canine tumors, hindering the development of similar immunotherapies for dogs.
Purpose of the Study:
- To investigate the expression of PD-L1 in various canine tumor cell lines and tissues.
- To identify canine tumor types that may benefit from anti-PD-1 and anti-PD-L1 therapies.
Main Methods:
- Analyzed PD-L1 expression in 31 canine tumor cell lines using flow cytometry and western blotting.
- Utilized immunohistochemistry to examine PD-L1 expression in canine tumor and normal tissue specimens.
Main Results:
- PD-L1 was detected intracellularly in nearly all canine tumor cell lines, with surface expression in only a few.
- Immunohistochemistry revealed frequent PD-L1 expression in malignant melanoma, mammary gland tumors, mast cell tumors, and lymphoma.
- Lower frequencies of PD-L1 expression were observed in soft-tissue sarcoma and hemangiosarcoma; some normal tissues also showed PD-L1 expression.
Conclusions:
- Canine tumors exhibiting PD-L1 expression are identified as potential candidates for anti-PD-1 and anti-PD-L1 immunotherapy.
- This study provides crucial information for advancing canine cancer immunotherapy strategies.
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