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Aging01:26

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Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
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Basal neutrophil function in human aging: Implications in endothelial cell adhesion.

Joes Nogueira-Neto1, André S C Cardoso1, Hugo P Monteiro2

  • 1Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil.

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|April 26, 2016
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Aging increases neutrophil inflammation markers, including higher reactive oxygen species and nitric oxide production. Elderly neutrophils show increased adhesion to endothelial cells, suggesting a role in age-related vascular damage.

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agingcell functionendothelial implications

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Area of Science:

  • Immunology
  • Gerontology
  • Vascular Biology

Background:

  • Aging is associated with a chronic, low-grade inflammatory state, termed 'inflammaging'.
  • Neutrophils play a critical role in inflammation and immune responses.
  • Age-related changes in neutrophil function may contribute to increased disease risk in the elderly.

Purpose of the Study:

  • To compare key functional and inflammatory parameters of neutrophils from young and elderly human volunteers.
  • To investigate age-associated alterations in neutrophil-mediated inflammation and endothelial cell interactions.

Main Methods:

  • Collected blood samples from young (18-30 years) and elderly (65-80 years) donors.
  • Measured plasma high-sensitivity C-reactive protein (hs-CRP) as an inflammatory marker.
  • Assessed non-stimulated neutrophil production of reactive oxygen species (ROS) and nitric oxide (NO).
  • Quantified expression of neutrophil adhesion molecules (CD11a, CD11b, CD18, CD62).
  • Evaluated neutrophil adhesion to endothelial cells in vitro.

Main Results:

  • Elderly individuals had higher plasma hs-CRP levels.
  • Non-stimulated neutrophils from aged donors exhibited significantly higher production of ROS (38% increase) and NO (29% increase).
  • CD11b expression was elevated on neutrophils from the aged group, while other adhesion molecules showed no significant difference.
  • Neutrophils from elderly donors demonstrated a 69% greater adhesion to endothelial cells in vitro.

Conclusions:

  • Aging is associated with enhanced pro-inflammatory status in neutrophils, characterized by increased ROS and NO production.
  • Elevated neutrophil adhesion to endothelial cells in aging may contribute to vascular damage.
  • These findings suggest a disrupted intracellular redox balance in neutrophils during aging, altering their function and potentially promoting vascular pathology.