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Related Experiment Video

Updated: Mar 22, 2026

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

Published on: January 15, 2014

Tuning Ice Nucleation with Supercharged Polypeptides.

Huige Yang^1,2, Chao Ma³, Kaiyong Li⁴

¹Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.

Advanced Materials (Deerfield Beach, Fla.)

|April 28, 2016

Summary

This summary is machine-generated.

Supercharged unfolded polypeptides (SUPs) control ice nucleation. Positively charged SUPs promote ice formation, while negatively charged ones inhibit it, offering tunable ice control.

Keywords:

electrostatic energy density ice nucleation interfacial water nucleation barrier supercharged polypeptides

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Last Updated: Mar 22, 2026

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

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Published on: January 15, 2014

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Area of Science:

Biophysics
Materials Science
Physical Chemistry

Background:

Ice nucleation is critical in various natural and industrial processes.
Controlling ice nucleation is essential for applications ranging from cryopreservation to weather modification.
Supercharged unfolded polypeptides (SUPs) offer a novel platform for modulating ice formation.

Purpose of the Study:

To investigate the role of charge and charge density of SUPs in controlling ice nucleation.
To determine how altering SUP properties affects ice formation.
To establish SUPs as tunable agents for ice nucleation modulation.

Main Methods:

Synthesis and characterization of SUPs with varying charges and charge densities.
Experimental setup to monitor ice nucleation in the presence of SUPs.
Analysis of ice crystal formation and growth dynamics.

Main Results:

Positively charged SUPs significantly facilitate ice nucleation.
Negatively charged SUPs effectively suppress ice nucleation.
The charge density of the SUP backbone provides an additional tunable parameter for ice nucleation control.

Conclusions:

SUPs can be engineered to either promote or inhibit ice nucleation.
The charge characteristics of SUPs are key determinants of their ice nucleation activity.
SUPs represent a promising class of biomolecules for precise control over ice formation.