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Active nano/microbilayer hemostatic agents for diabetic rat bleeding model
Zeynep Karahaliloğlu1, Murat Demirbilek2, İbrahim Ulusoy3
1Department of Biology, Faculty of Science and Arts, Aksaray University, Aksaray, Turkey.
Summary
A novel nano/microbilayer hemostatic dressing effectively controlled bleeding in diabetic rats. This dressing, utilizing chitosan, bacterial cellulose, and silk fibroin, shows promise for surgical hemostasis in diabetic patients.
Area of Science:
- Biomaterials Science
- Hemostasis Research
- Diabetic Complications Management
Background:
- Diabetes mellitus significantly elevates cardiovascular risk due to hemostatic system abnormalities.
- Diabetic patients face heightened risks of bleeding complications during surgical procedures.
- Effective hemostasis is crucial for managing surgical risks in diabetic individuals.
Purpose of the Study:
- To evaluate a fabricated nano/microbilayer hemostatic dressing for bleeding control in diabetic subjects.
- To assess the hemostatic efficiency of a novel dressing in a diabetic rat model.
- To investigate the potential of advanced biomaterials in managing surgical bleeding in diabetes.
Main Methods:
- Preparation of a nano/microbilayer hemostatic dressing with chitosan (CTS), bacterial cellulose (BC), vitamin K (Vit K), protamine sulfate (PS), kaolin (Kao), and silk fibroin (SF) or SF/phosphatidylcholine (PC).
- Evaluation of hemostatic performance using a diabetic rat femoral artery injury model, measuring bleeding time, blood loss, and mortality rate.
- Assessment of blood absorption capacity and coagulation time of the prepared dressings compared to gauze.
Main Results:
- The SF-coated PS/BC/CTS dressing demonstrated significantly higher blood absorption (2214 ± 56%) compared to gauze (247 ± 5%).
- Vit K-reinforced (138 s) and SF-coated BC/CTS (144 s) dressings exhibited rapid coagulation times.
- In vivo studies confirmed significant hemostatic plug formation, particularly with the PS/BC/CTS dressing.
Conclusions:
- The developed hemostatic dressings, especially those incorporating protamine sulfate, show remarkable efficacy in controlling bleeding in a diabetic rat model.
- The dressings' high positive charge and porosity facilitate rapid swelling and promote platelet and red blood cell accumulation via electrostatic interactions.
- This study highlights the potential of advanced nano/microbilayer hemostatic dressings for improving surgical outcomes in diabetic patients.
